山形大学紀要(医学) 第35巻 第2号(2017)90-100

The bone regeneration using bone marrow-derived mesenchymal stem cell with recombinant human bone morphogenetic protein-2 in allogeneic repair model of femoral segmental defect of rats

Mitsuo Takano, Junichi Hashimoto, Hiroyuki Tsuchida, Michiaki Takagi


 Treatment of a large bone defect is a difficult problem especially in modern aging society. Mesenchymal stem cells (MSCs) obtained from bone marrow are a suitable resource for bone regeneration. We performed a transplant of MSCs along with recombinant human bone morphogenetic protein 2 (rhBMP-2) in combination with an allogeneic bone marrow stem cell transplant in a rat model of a femoral segmental defect and observed sufficient bone formation. A single dose or 2-week administration of an immunosuppressive drug was necessary to ensure successful bone formation by the allogeneic stem cell transplant. Equivalent bone formation was attained in all groups 6 weeks after the transplant. As for the persistent administration of the immunosuppressive drug, it was found to be unnecessary because almost all transplanted allogeneic stem cells were absorbed during 8 weeks after the transplant.


Key words : mesenchymal stem cell, MSC, rhBMP-2, bone regeneration, allogeneic transplant, immunosuppressive drug





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